6M2D

MUL1-RING domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2013-06-13
Detector	ADSC QUANTUM 270
Wavelength(s)	1.2837
Spacegroup name	P 21 21 21
Unit cell lengths	59.226, 66.593, 68.271
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.671 - 1.795
R-factor	0.1744
Rwork	0.172
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t6p
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	10.000
High resolution limit [Å]	1.795	1.800
Rmerge	0.080
Rpim	0.026	0.026
Number of reflections	25501	25501
<I/σ(I)>	24.5
Completeness [%]	96.8
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.4 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3350