6M1X
Crystal structure of Phosphoserine Phosphatase in complex with 3-Phosphoglyceric Acid from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 82 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-02-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.976, 112.388, 177.428 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.870 - 2.480 |
| R-factor | 0.2299 |
| Rwork | 0.228 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zkk |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.619 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.870 | 2.590 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.506 | |
| Number of reflections | 33163 | 3206 |
| <I/σ(I)> | 10.83 | |
| Completeness [%] | 96.1 | |
| Redundancy | 4.1 | |
| CC(1/2) | 1.000 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M MMT buffer pH-4.0, PEG-1500 (20%), 0.2 M Ammonium sulphate |
