6M1B
A new V27M variant of beta 2 microglobulin induced amyloidosis in a patient with long-term hemodialysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.260, 28.900, 54.900 |
| Unit cell angles | 90.00, 121.68, 90.00 |
Refinement procedure
| Resolution | 32.860 - 1.880 |
| Rwork | 0.205 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxf |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.122 | 0.892 |
| Number of reflections | 14734 | 1126 |
| <I/σ(I)> | 5.69 | 1.47 |
| Completeness [%] | 89.3 | 90.8 |
| Redundancy | 2.1 | |
| CC(1/2) | 0.991 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 4000, ammonium acetate, glycerol, Phosphate buffer |
