6M0Q

Hydroxylamine oxidoreductase from Nitrosomonas europaea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-06
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	141.230, 141.910, 213.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.180 - 1.990
R-factor	0.1644
Rwork	0.163
R-free	0.19990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n4n
RMSD bond length	0.011
RMSD bond angle	1.444
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	2.110
High resolution limit [Å]	1.990	1.990
Rmeas	0.214	1.490
Number of reflections	567408	91130
<I/σ(I)>	8.38
Completeness [%]	99.8
Redundancy	7
CC(1/2)	0.995	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	293	MES-NaOH, PEG 400, potassium nitrate