6M09

The ligand-free structure of the chloroplast protein At3g03890

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	59.930, 79.964, 80.151
Unit cell angles	99.63, 109.80, 104.48

Refinement procedure

Resolution	37.763 - 2.101
R-factor	0.2016
Rwork	0.200
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n7r
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.12)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.047	0.548
Rmeas	0.066	0.775
Rpim	0.047	0.548
Number of reflections	73700	7308
<I/σ(I)>	17.1
Completeness [%]	97.2	96.6
Redundancy	2
CC(1/2)	0.989	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	289	ammonium acetate, trisodium citrate, PEG 4000