6LZI
Aquifex aeolicus MutL ATPase domain complexed with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-09 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.017, 42.906, 53.845 |
| Unit cell angles | 80.98, 71.52, 77.71 |
Refinement procedure
| Resolution | 41.727 - 1.691 |
| R-factor | 0.205044957344 |
| Rwork | 0.202 |
| R-free | 0.24257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x9y |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.733 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (v1.10.1) |
| Refinement software | PHENIX (v1.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.064 | 0.750 |
| Rpim | 0.041 | 0.430 |
| Number of reflections | 29323 | 2936 |
| <I/σ(I)> | 24.3 | 1.86 |
| Completeness [%] | 92.0 | 94.9 |
| Redundancy | 11.2 | 7.2 |
| CC(1/2) | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 50 mM tris-HCl, 50 mM NaCl, 5 mM MgCl2, 5 mM ADP |
