6LZ7

Tetrameric structure of ZmCRY1a PHR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-12-01
Detector	MAR555 FLAT PANEL
Wavelength(s)	0.9785
Spacegroup name	P 62 2 2
Unit cell lengths	154.023, 154.023, 141.072
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.601 - 3.599
R-factor	0.236119008145
Rwork	0.232
R-free	0.27349
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u3c
RMSD bond length	0.015
RMSD bond angle	1.698
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.610	3.728
High resolution limit [Å]	3.599	3.599
Number of reflections	15933	596
<I/σ(I)>	11
Completeness [%]	99.9
Redundancy	15.1
CC(1/2)	0.990	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20%(w/v) PEG 3350, 0.2M sodium thiocyanate