6LZ2

Crystal structure of a thermostable green fluorescent protein (TGP) with a synthetic nanobody (Sb44)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979300
Spacegroup name	P 21 21 21
Unit cell lengths	51.610, 83.486, 184.562
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.040 - 2.030
R-factor	0.1763
Rwork	0.175
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tza 5m13
RMSD bond length	0.007
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.530	2.090
High resolution limit [Å]	2.030	2.030
Rmerge	0.127	1.694
Rpim	0.037	0.504
Number of reflections	52046	3497
<I/σ(I)>	13.9	1.6
Completeness [%]	99.3	91.3
Redundancy	13.1	11.9
CC(1/2)	0.999	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M ammonium acetate, 25 %(w/v) polyethylene glycol 3350, 0.1M Bis-Tris pH 6.5