6LYW

Structural insight into the biological functions of Arabidopsis thaliana ACHT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	102.686, 100.560, 92.801
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.403 - 1.700
R-factor	0.1534
Rwork	0.152
R-free	0.17250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zzx
RMSD bond length	0.007
RMSD bond angle	1.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.064	0.032	0.695
Rmeas	0.072	0.036	0.775
Rpim	0.031	0.016	0.334
Total number of observations	253890
Number of reflections	52610	5327	5231
<I/σ(I)>	9.7
Completeness [%]	99.4	96.2	100
Redundancy	4.8	4.6	4.9
CC(1/2)		0.998	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	ammonium sulfate, isopropanol,agarose