6LYI

Crystal structure of a N-methyltransferase CkTbS from Camellia assamica var. kucha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9798
Spacegroup name	P 65
Unit cell lengths	144.768, 144.768, 76.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.386 - 2.490
R-factor	0.1859
Rwork	0.183
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2efj
RMSD bond length	0.006
RMSD bond angle	1.106
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.530
High resolution limit [Å]	2.490	2.490
Rmerge		2.000
Number of reflections	62522	34000
<I/σ(I)>	28.5
Completeness [%]	99.9
Redundancy	19.7
CC(1/2)	0.999	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.1 M MES (pH 6.0), 0.2 M Li2SO4, 20% PEG 4000