6LYF

Crystal structure of the mouse endonuclease EndoG(H138A/Se-Met)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	193
Detector technology	CCD
Collection date	2010-04-13
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	72.200, 81.880, 88.657
Unit cell angles	90.00, 97.59, 90.00

Refinement procedure

Resolution	48.120 - 2.800
R-factor	0.2015
Rwork	0.199
R-free	0.25690
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.065
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.120	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	56219	28825
<I/σ(I)>	13.5
Completeness [%]	99.7
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	200mM NaCl, 1mM CdCl2, 100mM CHES, 10mM CTAB, 5% glycerol, 5% MPD