6LYF
Crystal structure of the mouse endonuclease EndoG(H138A/Se-Met)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2010-04-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.200, 81.880, 88.657 |
Unit cell angles | 90.00, 97.59, 90.00 |
Refinement procedure
Resolution | 48.120 - 2.800 |
R-factor | 0.2015 |
Rwork | 0.199 |
R-free | 0.25690 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.065 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.120 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 56219 | 28825 |
<I/σ(I)> | 13.5 | |
Completeness [%] | 99.7 | |
Redundancy | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 200mM NaCl, 1mM CdCl2, 100mM CHES, 10mM CTAB, 5% glycerol, 5% MPD |