6LXQ
TvCyP2 in apo form 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.307, 54.643, 59.916 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.150 - 1.850 |
| R-factor | 0.15 |
| Rwork | 0.146 |
| R-free | 0.18270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yba |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.150 | 1.921 |
| High resolution limit [Å] | 1.850 | 1.855 |
| Number of reflections | 14941 | 1076 |
| <I/σ(I)> | 23.9 | 3.1 |
| Completeness [%] | 99.7 | 97.8 |
| Redundancy | 4.6 | |
| CC(1/2) | 0.981 | 0.924 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 288 | 0.2 M di-Ammonium hydrogen Phosphate, 20% w/v PEG 3350 |
