6LXM

Crystal structure of C-terminal DNA-binding domain of Escherichia coli OmpR as a domain-swapped dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.975
Spacegroup name	P 31 2 1
Unit cell lengths	98.683, 98.683, 92.717
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.450 - 2.412
R-factor	0.1857
Rwork	0.182
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	solved structure
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.450	2.490
High resolution limit [Å]	2.410	2.410
Rmerge	0.075
Number of reflections	19976	19976
<I/σ(I)>	26.1
Completeness [%]	100.0
Redundancy	7.2
CC(1/2)	0.974	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	0.1 M Potassium phosphate monobasic/sodium phosphate diabasic pH6.2 2.5M NaCl