6LWZ
Crystal structure of Laterosporulin10, bacteriocin produced by Brevibacillus sp. strain SKDU10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-06-06 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 22.280, 29.340, 32.150 |
Unit cell angles | 110.62, 89.99, 104.94 |
Refinement procedure
Resolution | 29.940 - 2.200 |
R-factor | 0.2237 |
Rwork | 0.224 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ozk |
RMSD bond length | 0.021 |
RMSD bond angle | 2.184 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.940 | 26.399 | 2.320 |
High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
Rmerge | 0.062 | 0.493 | |
Rmeas | 0.141 | 0.082 | 0.628 |
Rpim | 0.087 | 0.053 | 0.384 |
Total number of observations | 278 | 1191 | |
Number of reflections | 3487 | 116 | 473 |
<I/σ(I)> | 6.5 | 14.6 | 2.1 |
Completeness [%] | 94.1 | 97.1 | 90.3 |
Redundancy | 2.5 | 2.4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M calcium chloride, 0.1 M sodium cacodylate pH 5.5, 12 % PEG w/v 8000 and 3 % w/v Trimethylamine N-oxide dihydrate |