6LWT

Crystal structure of Staphylococcal Superantigen-Like protein 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	41.243, 71.211, 140.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.900
R-factor	0.2111
Rwork	0.209
R-free	0.24620
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.352
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.330	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.090	0.079	0.294
Rmeas	0.098	0.087	0.320
Rpim	0.039	0.036	0.125
Number of reflections	33517	1846	1644
<I/σ(I)>	9
Completeness [%]	100.0	99.9	100
Redundancy	6.4	6	6.6
CC(1/2)		0.991	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	2.1 M DL-Malic acid, pH 7.0