6LVV

N, N-dimethylformamidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0053
Spacegroup name	P 1 21 1
Unit cell lengths	160.514, 142.553, 181.488
Unit cell angles	90.00, 92.92, 90.00

Refinement procedure

Resolution	49.470 - 2.800
R-factor	0.2268
Rwork	0.227
R-free	0.26660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lvc
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18rc6_3830)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.470	2.870
High resolution limit [Å]	2.800	2.800
Rpim		0.789
Number of reflections	200660	9942
<I/σ(I)>	4.44
Completeness [%]	99.8	99.9
Redundancy	2
CC(1/2)	0.930	0.321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	291	PEG 3350, 16%; Tris-HCl buffer pH 7.2, 50 mM, NaCl 200 mM; KCl 200 mM, Protein 5 mg/ml.