6LVR

Crystal structure of the PPR domain of Arabidopsis thaliana protein-only RNase P 1 (PRORP1) in complex with tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	84.480, 131.701, 155.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.312 - 2.850
Rwork	0.235
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g24 1ehz
RMSD bond length	0.027
RMSD bond angle	1.606
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rmeas	0.124	1.075
Rpim	0.055	0.480
Number of reflections	42492	2074
<I/σ(I)>	13.8	1.56
Completeness [%]	99.9	100
Redundancy	5	4.8
CC(1/2)		0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1.5 M sodium citrate