6LVP
Enoyl-CoA hydratase (HyECH) from Hymenobacter sp. PAMC 26628
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2019-04-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 128.306, 128.306, 102.975 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.570 - 2.690 |
R-factor | 0.1907 |
Rwork | 0.188 |
R-free | 0.23740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zai |
RMSD bond length | 0.008 |
RMSD bond angle | 0.981 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.690 | 2.690 |
Number of reflections | 24538 | 1203 |
<I/σ(I)> | 49.7 | |
Completeness [%] | 100.0 | |
Redundancy | 14.5 | |
CC(1/2) | 1.000 | 0.981 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 0.16M magnesium acetate, 0.08M sodium cacodylate (pH 6.5), 16% (w/v) polyethylene glycol 8000, 20% (v/v) glycerol |