6LVP

Enoyl-CoA hydratase (HyECH) from Hymenobacter sp. PAMC 26628

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	200
Detector technology	CCD
Collection date	2019-04-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 42 21 2
Unit cell lengths	128.306, 128.306, 102.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.570 - 2.690
R-factor	0.1907
Rwork	0.188
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zai
RMSD bond length	0.008
RMSD bond angle	0.981
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.690	2.690
Number of reflections	24538	1203
<I/σ(I)>	49.7
Completeness [%]	100.0
Redundancy	14.5
CC(1/2)	1.000	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.16M magnesium acetate, 0.08M sodium cacodylate (pH 6.5), 16% (w/v) polyethylene glycol 8000, 20% (v/v) glycerol