6LVN

Structure of the 2019-nCoV HR2 Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-04
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	28.195, 49.729, 89.576
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.600 - 2.470
Rwork	0.215
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wyy
RMSD bond length	0.004
RMSD bond angle	0.726
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.894	2.558
High resolution limit [Å]	2.469	2.470
Rmerge	0.132
Number of reflections	4862	456
<I/σ(I)>	19.06
Completeness [%]	99.2
Redundancy	13.5
CC(1/2)	1.000	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Tris HCl, pH 8.5, 25% PEG3350