6LVH

Crystal structure of methionine aminopeptidase from Pyrococcus furiosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS IV
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-10-29
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 62
Unit cell lengths	133.658, 133.658, 62.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.450 - 3.200
R-factor	0.1646
Rwork	0.162
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wkm
RMSD bond length	0.008
RMSD bond angle	1.740
Data reduction software	DENZO (2.3.1)
Data scaling software	SCALEPACK (2.2.0)
Phasing software	MOLREP (11.7.01)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	3.310
High resolution limit [Å]	3.200	6.850	3.200
Rmerge	0.073	0.036	0.398
Rmeas	0.086	0.043	0.496
Rpim	0.045	0.022	0.291
Total number of observations	36517
Number of reflections	10354	1103	896
<I/σ(I)>	10.8
Completeness [%]	97.0	98.8	86
Redundancy	3.5	3.7	2.5
CC(1/2)		0.998	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	0.1 M Sodium acetate, 2M NaCl, 5% glycerol