6LV0
Crystal structure of the soluble domain of the multiple peptide resistance factor (MprF) from Rhizobium tropici
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2013-11-30 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 83.588, 95.793, 211.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.207 - 1.998 |
| R-factor | 0.1699 |
| Rwork | 0.168 |
| R-free | 0.19840 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.849 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.998 | 1.998 |
| Rmerge | 0.047 | 0.195 |
| Number of reflections | 57555 | 5564 |
| <I/σ(I)> | 35.5 | 8.3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.1 M NaAc, pH 6.0, 16% PEG3350 |
