6LUS

Crystal structure of the Mengla Virus VP30 C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97852
Spacegroup name	P 32 1 2
Unit cell lengths	73.886, 73.886, 53.405
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.401 - 1.400
Rwork	0.177
R-free	0.18220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t3w
RMSD bond length	0.017
RMSD bond angle	1.837
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Number of reflections	32952	1628
<I/σ(I)>	56.96
Completeness [%]	100.0
Redundancy	19.9
CC(1/2)	0.995	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	Citric acid, Polyethylene glycol 3350