6LU2

Crystal structure of a substrate binding protein from Microbacterium hydrocarbonoxydans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-27
Detector	RAYONIX MX225HE
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	49.263, 78.443, 123.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.551 - 1.750
Rwork	0.194
R-free	0.20640
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.465
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.092	1.100
Number of reflections	48691	2301
<I/σ(I)>	50.6	2.8
Completeness [%]	99.3	95.7
Redundancy	13.2	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium acetate 0.1 M sodium cacodylate, pH 7.5 30% (w/v) PEG8000