6LTZ

Induced DNA bending by unique dimerization of HigA antitoxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	I 4
Unit cell lengths	84.232, 84.232, 61.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.113 - 1.973
R-factor	0.1906
Rwork	0.189
R-free	0.23370
Structure solution method	SAD
Data reduction software	DIFDAT
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.970	1.970
Rmeas	0.060	0.194
Number of reflections	15201	762
<I/σ(I)>	41.5
Completeness [%]	100.0
Redundancy	3.5
CC(1/2)		0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1 M ammonium sulfate, 0.1 M HEPES, 0.5% w/v PEG8000