6LTM
The dimeric structure of G80A/H81A/H82A myoglobin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-11 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.225, 62.841, 81.532 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.770 - 1.650 |
R-factor | 0.19363 |
Rwork | 0.192 |
R-free | 0.23050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vm9 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.933 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.092 | |
Number of reflections | 34523 | 1682 |
<I/σ(I)> | 23.3 | |
Completeness [%] | 99.9 | |
Redundancy | 6.5 | |
CC(1/2) | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M Tris-HCl buffer, 0.1M sodium acetate, 12.5% (w/v) PEG 6000 |