6LTL
The dimeric structure of G80A myoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-25 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.902, 63.060, 83.514 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.250 |
| R-factor | 0.22226 |
| Rwork | 0.221 |
| R-free | 0.24544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vm9 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.070 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.096 | 0.918 |
| Number of reflections | 85063 | 4107 |
| <I/σ(I)> | 23.9 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.849 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M Tris-HCl buffer, 0.1M sodium acetate, 10% (w/v) PEG 6000 |
