6LT9

The crystal structure of diamondback moth ryanodine receptor SPRY1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9792
Spacegroup name	P 43
Unit cell lengths	64.893, 64.893, 95.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.942 - 1.801
R-factor	0.1883
Rwork	0.186
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c33
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.102	0.052	0.365
Rmeas	0.112	0.057	0.462
Rpim	0.044	0.022	0.277
Total number of observations	212691
Number of reflections	36452	1897	1655
<I/σ(I)>	4.7
Completeness [%]	99.3	100	90.6
Redundancy	5.8	6.5	2.3
CC(1/2)		0.998	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	2M Ammonium, 0.2M sodium chloride, 0.1M sodium cacodylate, ph 6.5.