6LT7

Crystal structure of human RPP20-RPP25 proteins in complex with the P3 domain of lncRNA RMRP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-07-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97776
Spacegroup name	P 32
Unit cell lengths	144.168, 144.168, 74.223
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.600 - 2.700
R-factor	0.1938
Rwork	0.192
R-free	0.23850
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.813
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.187	0.179	0.803
Rmeas	0.198	0.190	0.857
Rpim	0.062	0.063	0.293
Total number of observations	482821
Number of reflections	47259	2368	2380
<I/σ(I)>	4.1
Completeness [%]	100.0	100	99.7
Redundancy	10.2	10.2	8.1
CC(1/2)		0.920	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	277	20% of PEG 3350, 0.2 M ammonium tartrate dibasic, pH 6.6