6LSU
Crystal structure of Uso1-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.376, 104.376, 231.842 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.790 - 2.700 |
| R-factor | 0.1994 |
| Rwork | 0.198 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lst |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.742 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
| Rmerge | 0.095 | 0.062 | 0.913 |
| Rmeas | 0.104 | 0.070 | 0.998 |
| Rpim | 0.043 | 0.032 | 0.398 |
| Total number of observations | 243939 | ||
| Number of reflections | 41061 | 2168 | 2035 |
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 99.8 | 95.8 | 100 |
| Redundancy | 5.9 | 4.8 | 6.1 |
| CC(1/2) | 0.993 | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Bis-Tris: HCl pH 6.0, 2M Sodium Formate |
