6LSB

Crystal Structure of DPF domain of MOZ in complex with H3K14bz peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	48.187, 48.187, 116.848
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.300 - 2.000
R-factor	0.1626
Rwork	0.158
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b76
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.091	0.048	0.412
Rmeas	0.101	0.054	0.447
Rpim	0.043	0.024	0.169
Total number of observations	53278
Number of reflections	10923	617	528
<I/σ(I)>	10.9
Completeness [%]	97.7	95.7	98.7
Redundancy	4.9	4.5	5.5
CC(1/2)		0.997	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M HEPES pH 7.5, 1.4 M sodium citrate tribasic dehydrate.