6LS3
cubic-shaped crystal of TmFtn mutant-T4FY stimulated by Mg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2019-11-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | H 3 2 |
Unit cell lengths | 171.737, 171.737, 384.289 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 138.703 - 2.815 |
R-factor | 0.1895 |
Rwork | 0.187 |
R-free | 0.24270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vlg |
RMSD bond length | 0.009 |
RMSD bond angle | 0.952 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 138.703 | 2.961 |
High resolution limit [Å] | 2.815 | 2.815 |
Number of reflections | 50424 | 5252 |
<I/σ(I)> | 4.64 | |
Completeness [%] | 95.0 | |
Redundancy | 5 | |
CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Potassium phosphate monobasic, Sodium phosphate dibasic pH 6.2, and 2500 mM Sodium chloride, 60 mM MgCl2 |