6LS0

rod-shaped crystal of TmFtn mutant-T4FY stimulited by PLL15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	273
Detector technology	CCD
Collection date	2019-11-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	85.480, 94.120, 109.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.215 - 1.870
R-factor	0.226
Rwork	0.225
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.215	1.937
High resolution limit [Å]	1.870	1.870
Number of reflections	72846	7030
<I/σ(I)>	8.5
Completeness [%]	99.0
Redundancy	1
CC(1/2)	0.996	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Tris base/Hydrochloric acid pH 8.5,