6LRY

Crystal structure of human endothelin ETB receptor in complex with sarafotoxin S6b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 2 21 21
Unit cell lengths	61.120, 82.280, 166.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.297 - 3.000
R-factor	0.2714
Rwork	0.270
R-free	0.30370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6igk
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	3.107
High resolution limit [Å]	3.000	3.000
Number of reflections	17494	1724
<I/σ(I)>	9.06
Completeness [%]	99.8
Redundancy	19.3
CC(1/2)	0.994	0.545

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5	293	PEG 600, Ammonium sulfate