6LRX

Marsupenaeus japonicus ferritin mutant(T158H)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	273.15
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 4 3 2
Unit cell lengths	117.186, 117.186, 117.186
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.621 - 1.702
R-factor	0.1495
Rwork	0.148
R-free	0.17440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a4u
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.197	0.069	1.590
Rmeas	0.199	0.070	1.618
Rpim	0.033	0.012	0.294
Total number of observations	1110087
Number of reflections	30779	3349	3001
<I/σ(I)>	2.9
Completeness [%]	100.0	100	100
Redundancy	36.1	34.9	29.6
CC(1/2)		0.999	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	2500 mM NaCl, 100 mM KH2PO3 / KH2PO3 (pH 8.0), 2 mM NiSO4