6LRW

Marsupenaeus japonicus ferritin mutant(T158H) pH 7.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	273.15
Detector technology	PIXEL
Collection date	2019-01-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 2 3
Unit cell lengths	117.114, 117.114, 117.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.1785
Rwork	0.176
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a4u
RMSD bond length	0.017
RMSD bond angle	1.755
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	117.110	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.142	0.054	0.482
Rmeas	0.146	0.056	0.497
Rpim	0.033	0.013	0.118
Number of reflections	21207	1143	1044
<I/σ(I)>	3.9
Completeness [%]	100.0	100	100
Redundancy	19.1	18	17.5
CC(1/2)		1.000	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	2500 mM NaCl, 100 mM KH2PO3 / KH2PO3 (pH 7.0)