6LRF
Crystal structure of unliganded AgrE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-07-15 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 187.647, 201.726, 99.593 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.592 - 2.055 |
R-factor | 0.208183254555 |
Rwork | 0.208 |
R-free | 0.24641 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.104 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 114936 | 10839 |
<I/σ(I)> | 12.24 | |
Completeness [%] | 98.2 | |
Redundancy | 6.6 | |
CC(1/2) | 0.989 | 0.437 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295.15 | sodium acetate, sodium citrate (pH5.5), PEG4000, glycerol, TCEP |