6LR7

Crystal structure of GFPuv complexed with the nanobody LaG16 at 1.67 Angstron resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	48.370, 41.780, 81.710
Unit cell angles	90.00, 91.89, 90.00

Refinement procedure

Resolution	42.220 - 1.670
R-factor	0.187
Rwork	0.185
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k1k 6ir6
RMSD bond length	0.007
RMSD bond angle	1.083
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12-2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.215	1.770
High resolution limit [Å]	1.670	1.670
Rmerge	0.109	0.843
Number of reflections	37938	37938
<I/σ(I)>	12.04	2.19
Completeness [%]	98.8	92.7
Redundancy	5.856	3.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.3M Sodium Chloride, 0.01M Tris 8.0, 27.5% w/v PEG