6LQO
EBV tegument protein BBRF2/BSRF1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-04-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978530 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.778, 161.848, 97.862 |
| Unit cell angles | 90.00, 100.78, 90.00 |
Refinement procedure
| Resolution | 44.335 - 3.091 |
| R-factor | 0.219494301854 |
| Rwork | 0.217 |
| R-free | 0.26934 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lqn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.095 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.070 | 3.280 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Number of reflections | 42196 | 6720 |
| <I/σ(I)> | 13.27 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 277.15 | 0.1 M magnesium acetate; 0.05 M MES pH 5.6; 20% MPD |
