6LQN
EBV tegument protein BBRF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.320, 61.570, 58.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.660 - 1.600 |
| R-factor | 0.166858938464 |
| Rwork | 0.166 |
| R-free | 0.18880 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.109 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.150 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.085 | 0.674 |
| Number of reflections | 66862 | 10656 |
| <I/σ(I)> | 13.39 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.09 M NPS (0.03 M NaNO3, 0.03 M Na2HPO4, 0.03 M (NH4)2SO4); 0.1 M MES/imidazole pH 6.5; 37.5% precipitants (12.5% PEG1000,12.5% PEG 3350,12.5% MPD) |
