6LQK

Crystal structure of honeybee RyR NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2019-01-13
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	36.054, 75.363, 143.878
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.682 - 2.499
R-factor	0.229
Rwork	0.223
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y9v
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.499	6.780	2.500
Rmerge	0.122	0.077	0.699
Rmeas	0.134	0.085	0.773
Rpim	0.054	0.035	0.322
Number of reflections	14282	819	710
<I/σ(I)>	4.8
Completeness [%]	99.8	99.4	98.7
Redundancy	6.1	5.5	5.4
CC(1/2)		0.997	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.2 M magnesium chloride hexahydrate, 34%-40% PEG 4000, 0.1 M Tris, pH 8.5-9.4