6LQC

Crystal structure of Cyclohexylamine Oxidase from Erythrobacteraceae bacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	82.591, 82.591, 175.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.300 - 1.880
R-factor	0.1727
Rwork	0.172
R-free	0.18950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i59
RMSD bond length	0.015
RMSD bond angle	1.660
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.880	1.880
Rmerge	0.112	0.761
Rpim	0.042	0.254
Number of reflections	50354	2452
<I/σ(I)>	41	3.7
Completeness [%]	100.0	100
Redundancy	25.6	24.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10 % PEG 8000,100 mM Potassium phosphate monobasic/ Sodium phosphate dibasic pH 6.0