6LPW

Structure of Spermidine disinapoyl transferases(SDT) from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	77
Detector technology	PIXEL
Collection date	2013-01-09
Detector	DECTRIS EIGER X 500K
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	60.989, 103.062, 135.411
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.161 - 2.401
R-factor	0.2021
Rwork	0.200
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g0b
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.490
Number of reflections	32701
<I/σ(I)>	15
Completeness [%]	95.8
Redundancy	3
CC(1/2)	0.090	0.090

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M MES (pH 6.5), 25% W/V PEG 4000 at 20 C