6LPS
Crystal structure of family 10 xylanase from Bacillus halodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-07-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.450, 42.353, 58.769 |
| Unit cell angles | 90.00, 98.59, 90.00 |
Refinement procedure
| Resolution | 39.168 - 2.214 |
| R-factor | 0.167369570685 |
| Rwork | 0.165 |
| R-free | 0.20912 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uwf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.819 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.073 | 0.219 |
| Number of reflections | 19247 | |
| <I/σ(I)> | 19.89 | 8.02 |
| Completeness [%] | 98.3 | 93.6 |
| Redundancy | 6.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2M Magnesium chloride, 0.1M Hepes pH 7, 20% PEG 6000 |
