6LP5

Structure of Sinonovacula constricta ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97892
Spacegroup name	I 4
Unit cell lengths	154.510, 154.510, 129.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.260 - 1.980
R-factor	0.1654
Rwork	0.163
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bg7
RMSD bond length	0.026
RMSD bond angle	2.184
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.260	2.010
High resolution limit [Å]	1.980	1.980
Number of reflections	697312	104802
<I/σ(I)>	38.5
Completeness [%]	99.3
Redundancy	6.7
CC(1/2)	0.980	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	1 uL of protein (10 mg/mL, 25 mM Tris-HCl, 150 mM NaCl, pH 8.0), 1 uL precipitant solution (1.0 M 1,6-hexanediol, 0.01 M cobalt (2+) chloride hexahydrate, 0.1 M sodium acetate trihydrate pH 4.6)