6LNH
Crystal structure of IDO from Bacillus thuringiensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-09-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.425, 120.965, 81.715 |
| Unit cell angles | 90.00, 108.11, 90.00 |
Refinement procedure
| Resolution | 24.860 - 2.340 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.26400 |
| Structure solution method | SIR |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.092 | 0.375 |
| Number of reflections | 52010 | 4513 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 97.9 | 85.7 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 18% PEG 3350, 0.1 M Bis-Tris, pH 5.6, Tacsimate pH 6.0 |
