6LND

Crystal structure of transposition protein TniQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9794
Spacegroup name	P 41 21 2
Unit cell lengths	66.277, 66.277, 178.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.001
R-factor	0.1944
Rwork	0.192
R-free	0.22830
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	AutoSol
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.096	0.061	0.702
Rmeas	0.100	0.063	0.727
Rpim	0.027	0.017	0.190
Number of reflections	51187	3048	2711
<I/σ(I)>	6.5
Completeness [%]	100.0	99.8	100
Redundancy	14.2	13.4	14.4
CC(1/2)		0.986	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.7	293	0.1 M Tris-HCl (pH 8.7), 2% Polyethylene glycol 8000 (w/v)