6LM2

Crystal structure of Zinc binding protein ZinT from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	55.810, 55.810, 153.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.355 - 2.130
R-factor	0.228154334809
Rwork	0.226
R-free	0.27471
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yxc
RMSD bond length	0.007
RMSD bond angle	0.863
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.130	2.190
High resolution limit [Å]	2.130	2.130
Number of reflections	14365	1138
<I/σ(I)>	17.4
Completeness [%]	99.9
Redundancy	24.6
CC(1/2)	0.998	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Zinc acetate, 0.1 M Sodium acetate pH 4.5, 10 %(w/v) PEG 3000