6LL5

Crystal structure of KpFtsZ (residues 11-316)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	37.120, 40.930, 44.200
Unit cell angles	89.22, 72.86, 73.44

Refinement procedure

Resolution	42.110 - 1.750
R-factor	0.1814
Rwork	0.179
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h5g
RMSD bond length	0.007
RMSD bond angle	0.945
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.110	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	22348	1826
<I/σ(I)>	5.41
Completeness [%]	93.2
Redundancy	3.4
CC(1/2)	0.941	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PCB buffer, PEG 1500