6LKX

The structure of PRRSV helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	93.623, 93.623, 357.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.812 - 2.998
Rwork	0.225
R-free	0.28070
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.571	3.106
High resolution limit [Å]	2.998	2.998
Number of reflections	32988	31009
<I/σ(I)>	51.29
Completeness [%]	100.0
Redundancy	23.7
CC(1/2)	0.975	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M HEPES pH7.5; 2.0 M NaCl