6LKT

Crystal structure of the Fab fragment of murine monoclonal antibody KH-1 against Human herpesvirus 6B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-29
Detector	RAYONIX MX-225
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.400, 120.000, 42.600
Unit cell angles	90.00, 97.00, 90.00

Refinement procedure

Resolution	39.106 - 1.800
R-factor	0.1665
Rwork	0.165
R-free	0.19630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ok0
RMSD bond length	0.007
RMSD bond angle	1.102
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.106	39.106	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.086	0.026	0.622
Rmeas	0.093	0.028	0.674
Number of reflections	36273	416	2613
<I/σ(I)>	17.97	55.79	3.25
Completeness [%]	99.7	98.8	97.4
Redundancy	7.453	6.822	6.765
CC(1/2)	0.999	0.999	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	277	100 mM Tris-HCl, pH 8.8, polyethylene glycol 3350 22.5% (w/v)